《植物生理学报》 2009, 45(11): 1065-1069

甘薯质体中的乙酰辅酶A 羧化酶亚基基因accD 的克隆与序列分析

王玉华*, 郝建国, 贾敬芬

西北大学生命科学学院, 陕西省生物技术重点实验室, 西部资源生物与现代生物技术教育部重点实验室, 西安 710069

通信作者：王玉华；E-mail: wangyh@nwu.edu.cn；Tel: 029-88303484

摘　要：

依据烟草质体全基因组序列设计引物, 以甘薯质体基因组 DNA 为模板, PCR 扩增包含质体 accD 基因完整编码区在 内的一段序列(GenBank 登录号为 GQ395771)。序列分析表明: 该片段全长为 2 209 bp, 包括 1 548 bp 的 accD 基因编码序列, 推测编码 515 个氨基酸的蛋白质, 该蛋白序列具有异质型 β-CT 中保守的锌指结构和 C 末端 5 个基元。同时绘制了该 DNA 片段的限制性酶切图谱。相似性比较显示, 甘薯 accD 基因与大豆、马铃薯、拟南芥、人参、莴苣、葡萄、海岛棉、甘蓝、 辣椒、菠菜、番茄和烟草的 accD 基因核苷酸相似性为 72%~87%, 氨基酸相似性为 58%~83%。

关键词：甘薯; 乙酰辅酶 A 羧化酶(ACCase); accD 基因; 克隆

收稿：2009-07-31   修定：2009-09-30

资助：陕西省教育厅专项(09JK777)、国家自然科学基金(30900914 和30671082)、西北大学西部资源生物与生物技术重点实验室开放基金。

Cloning and Sequence Analyses of Plastid-Encoded Acetyl-CoA Carboxylase Subunit Gene accD of Sweet Potato [Ipomoea batatas (L.) Lam.]

WANG Yu-Hua*, HAO Jian-Guo, JIA Jing-Fen

College of Life Sciences, Northwest University, Shaanxi Provincial Key Laboratory of Biotechnology, Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, Xi’an 710069, China

Corresponding author: WANG Yu-Hua; E-mail: wangyh@nwu.edu.cn; Tel: 029-88303484

Abstract:

The fragment containing plastid accD gene (GenBank accession number: GQ395771) encoding the carboxyltransferase β subunit of acetyl-CoA carboxylase (ACCase) was cloned from plastid genome DNA in sweet potato (Ipomoea batatas). Sequencing analysis indicated that sweet potato accD is 2 209 bp with an 1 548 bp open reading frame (ORF), encoding a 515-amino-acid polypeptide which contained the zinc finger structure and the C-terminal regions with five motifs. The restriction map of this cloned fragment was also established. The BLAST results showed that the homologies of this gene with soybean, potato, tobacco, Arabidopsis thaliana, ginseng, lettuce, grape, sea-island cotton, cabbage, pepper, spinach, tomato and tobacco were from 72% to 87%, and the similarities of the amino acid sequences were from 58% to 83%.

Key words: sweet potato (Ipomoea batatas); acetyl-CoA carboxylase (ACCase); accD gene; cloning